The camera’s autofocus functioned with impressive reliability and precision. In practical tests, even under challenging conditions, it consistently delivered absolute accuracy. Visual assessments confirmed that no further corrections could enhance the focus achieved by the autofocus system. Importantly, when an out-of-focus image appeared in the microscope itself, the camera’s extensive autofocus adjustment range allowed sharp images to appear on the monitor without requiring adjustments to the objective’s working distance.

To demonstrate this capability, we used the L 32/0.40 objective (long working distance) combined with a 10x eyepiece. After manually focusing on a slide in mid-position using autofocus, our tests revealed that the microscope’s focus could be adjusted by up to 8µm before autofocus failed to compensate adequately for out-of-focus images seen on screen—meaning it effectively managed an adjustment range of at least ±8µm around the set point.

This translates approximately to three times what might normally be expected from this objective/eyepiece combination regarding depth of field. Significantly, even when using non-corrected objectives like simple achromats known for edge softness, consistent specimen focus was maintained across practical tests while enabling selective autofocus control—adjusting only specific areas within the image frame as needed (Fig. 12).

Moreover, if one adjusts manually but then keeps settings unchanged slightly between observations via different views or magnifications, leveraging selective autofocus makes it straightforward to refocus precisely from center outward toward periphery with minimal effort.

Key Points:

  • Autofocus provides reliable performance even under challenging conditions.
  • Tested adjustment range allows for significant flexibility without manual intervention.
  • Works effectively across various objectives including non-corrected ones.
  • Offers precise control over specific regions within an image frame.

Last Updated: 2025-09-05 01:01:41